RNA

Part:BBa_K2148014:Design

Designed by: Ciara McCarthy   Group: iGEM16_Cambridge-JIC   (2016-10-12)


gRNA for CGG PAM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

2 Gs added after TSS to stabilise gRNA, following advice of N. Patron (Earlham Institute)


Source

Target sequence designed using Benchling software (https://benchling.com/crispr), scaffold sequence from Addgene (https://www.addgene.org/static/cms/files/hCRISPR_gRNA_Synthesis.pdf)

References